Signalling

Part:BBa_I756032:Design

Designed by: Omar Khan   Group: iGEM07_Bay_Area_RSI   (2007-10-25)


FcGammaRIIa-R131


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 952
    Illegal PstI site found at 551
    Illegal PstI site found at 874
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 551
    Illegal PstI site found at 874
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 335
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 952
    Illegal PstI site found at 551
    Illegal PstI site found at 874
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 952
    Illegal PstI site found at 551
    Illegal PstI site found at 874
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 795
    Illegal SapI site found at 466


Design Notes

Cloned into pcDNA3.1 Zeo (+) with EcoRV and XbaI. XbaI site at 3' end was continually destroyed. Problem with oligo accuracy suspected.

FCGamma can be cut out with 5'EcoR1 and 3' Xba.

Source

FcGammaRIIa-R131 from OpenBiosystems (Clone ID: 4704252)

References